Understanding the response of Desulfovibrio desulfuricans ATCC 27774 to the electron acceptors nitrate and sulfate - biosynthetic costs modulate substrate selection.
Identifieur interne : 000A46 ( Main/Exploration ); précédent : 000A45; suivant : 000A47Understanding the response of Desulfovibrio desulfuricans ATCC 27774 to the electron acceptors nitrate and sulfate - biosynthetic costs modulate substrate selection.
Auteurs : Joana R. Sousa [Portugal] ; Célia M. Silveira [Portugal] ; Pedro Fontes [Portugal] ; Catarina Roma-Rodrigues [Portugal] ; Alexandra R. Fernandes [Portugal] ; Gonzalez Van Driessche [Portugal] ; Bart Devreese [Belgique] ; Isabel Moura [Portugal] ; José J G. Moura [Portugal] ; M Gabriela Almeida [Portugal]Source :
- Biochimica et biophysica acta. Proteins and proteomics [ 1570-9639 ] ; 2017.
Descripteurs français
- KwdFr :
- Anaérobiose (génétique), Annotation de séquence moléculaire (MeSH), Desulfovibrio desulfuricans (croissance et développement), Desulfovibrio desulfuricans (effets des médicaments et des substances chimiques), Desulfovibrio desulfuricans (génétique), Desulfovibrio desulfuricans (métabolisme), Gene Ontology (MeSH), Milieux de culture (composition chimique), Milieux de culture (pharmacologie), Nitrate reductase (génétique), Nitrate reductase (métabolisme), Nitrates (métabolisme), Nitrates (pharmacologie), Nitrite reductases (génétique), Nitrite reductases (métabolisme), Oxydoréduction (MeSH), Protéines bactériennes (génétique), Protéines bactériennes (métabolisme), Protéome (génétique), Protéome (métabolisme), Régulation de l'expression des gènes bactériens (MeSH), Sulfates (métabolisme), Sulfates (pharmacologie), Transport d'électrons (MeSH), Voies et réseaux métaboliques (MeSH), Électrons (MeSH), Électrophorèse bidimensionnelle sur gel (MeSH).
- MESH :
- composition chimique : Milieux de culture.
- croissance et développement : Desulfovibrio desulfuricans.
- effets des médicaments et des substances chimiques : Desulfovibrio desulfuricans.
- génétique : Anaérobiose, Desulfovibrio desulfuricans, Nitrate reductase, Nitrite reductases, Protéines bactériennes, Protéome.
- métabolisme : Desulfovibrio desulfuricans, Nitrate reductase, Nitrates, Nitrite reductases, Protéines bactériennes, Protéome, Sulfates.
- pharmacologie : Milieux de culture, Nitrates, Sulfates.
- Annotation de séquence moléculaire, Gene Ontology, Oxydoréduction, Régulation de l'expression des gènes bactériens, Transport d'électrons, Voies et réseaux métaboliques, Électrons, Électrophorèse bidimensionnelle sur gel.
English descriptors
- KwdEn :
- Anaerobiosis (genetics), Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Culture Media (chemistry), Culture Media (pharmacology), Desulfovibrio desulfuricans (drug effects), Desulfovibrio desulfuricans (genetics), Desulfovibrio desulfuricans (growth & development), Desulfovibrio desulfuricans (metabolism), Electron Transport (MeSH), Electrons (MeSH), Electrophoresis, Gel, Two-Dimensional (MeSH), Gene Expression Regulation, Bacterial (MeSH), Gene Ontology (MeSH), Metabolic Networks and Pathways (MeSH), Molecular Sequence Annotation (MeSH), Nitrate Reductase (genetics), Nitrate Reductase (metabolism), Nitrates (metabolism), Nitrates (pharmacology), Nitrite Reductases (genetics), Nitrite Reductases (metabolism), Oxidation-Reduction (MeSH), Proteome (genetics), Proteome (metabolism), Sulfates (metabolism), Sulfates (pharmacology).
- MESH :
- chemical , chemistry : Culture Media.
- chemical , genetics : Bacterial Proteins, Nitrate Reductase, Nitrite Reductases, Proteome.
- drug effects : Desulfovibrio desulfuricans.
- genetics : Anaerobiosis, Desulfovibrio desulfuricans.
- growth & development : Desulfovibrio desulfuricans.
- chemical , metabolism : Bacterial Proteins, Desulfovibrio desulfuricans, Nitrate Reductase, Nitrates, Nitrite Reductases, Proteome, Sulfates.
- chemical , pharmacology : Culture Media, Nitrates, Sulfates.
- Electron Transport, Electrons, Electrophoresis, Gel, Two-Dimensional, Gene Expression Regulation, Bacterial, Gene Ontology, Metabolic Networks and Pathways, Molecular Sequence Annotation, Oxidation-Reduction.
Abstract
Sulfate-reducing bacteria (SRB) are a diverse group of anaerobic microorganisms that obtain their energy from dissimilatory sulfate reduction. Some SRB species have high respiratory versatility due to the possible use of alternative electron acceptors. A good example is Desulfovibrio desulfuricans ATCC 27774, which grows in the presence of nitrate (end product: ammonium) with higher rates and yields to those observed in sulfate containing medium (end product: sulfide). In this work, the mechanisms supporting the respiratory versatility of D. desulfuricans were unraveled through the analysis of the proteome of the bacterium under different experimental conditions. The most remarkable difference in the two-dimensional gel electrophoresis maps is the high number of spots exclusively represented in the nitrate medium. Most of the proteins with increase abundance are involved in the energy metabolism and the biosynthesis of amino acids (or proteins), especially those participating in ammonium assimilation processes. qPCR analysis performed during different stages of the bacterium's growth showed that the genes involved in nitrate and nitrite reduction (napA and nrfA, respectively) have different expressions profiles: while napA did not vary significantly, nrfA was highly expressed at a 6h time point. Nitrite levels measured along the growth curve revealed a peak at 3h. Thus, the initial consumption of nitrate and concomitant production of nitrite must induce nrfA expression. The activation of alternative mechanisms for energy production, aside several N-assimilation metabolisms and detoxification processes, solves potential survival problems in adapting to different environments and contributes to higher bacterial growth rates.
DOI: 10.1016/j.bbapap.2017.07.021
PubMed: 28847524
Affiliations:
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Moura</name><affiliation wicri:level="1"><nlm:affiliation>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica</wicri:regionArea><wicri:noRegion>2829-516 Monte de Caparica</wicri:noRegion></affiliation></author><author><name sortKey="Almeida, M Gabriela" sort="Almeida, M Gabriela" uniqKey="Almeida M" first="M Gabriela" last="Almeida">M Gabriela Almeida</name><affiliation wicri:level="1"><nlm:affiliation>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal; Centro de Investigação Interdisciplinar Egas Moniz (CiiEM), Instituto Superior de Ciências da Saúde Egas Moniz, Campus Universitário, Quinta da Granja, 2829-511 Caparica, Portugal. Electronic address: mg.almeida@fct.unl.pt.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal; Centro de Investigação Interdisciplinar Egas Moniz (CiiEM), Instituto Superior de Ciências da Saúde Egas Moniz, Campus Universitário, Quinta da Granja, 2829-511 Caparica</wicri:regionArea><wicri:noRegion>2829-511 Caparica</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28847524</idno><idno type="pmid">28847524</idno><idno type="doi">10.1016/j.bbapap.2017.07.021</idno><idno type="wicri:Area/Main/Corpus">000A87</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000A87</idno><idno type="wicri:Area/Main/Curation">000A87</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000A87</idno><idno type="wicri:Area/Main/Exploration">000A87</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Understanding the response of Desulfovibrio desulfuricans ATCC 27774 to the electron acceptors nitrate and sulfate - biosynthetic costs modulate substrate selection.</title><author><name sortKey="Sousa, Joana R" sort="Sousa, Joana R" uniqKey="Sousa J" first="Joana R" last="Sousa">Joana R. Sousa</name><affiliation wicri:level="1"><nlm:affiliation>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica</wicri:regionArea><wicri:noRegion>2829-516 Monte de Caparica</wicri:noRegion></affiliation></author><author><name sortKey="Silveira, Celia M" sort="Silveira, Celia M" uniqKey="Silveira C" first="Célia M" last="Silveira">Célia M. Silveira</name><affiliation wicri:level="1"><nlm:affiliation>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal; Instituto de Tecnologia Química e Biológica António Xavier, Universidade NOVA de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal; Instituto de Tecnologia Química e Biológica António Xavier, Universidade NOVA de Lisboa, Av. da República, 2780-157 Oeiras</wicri:regionArea><wicri:noRegion>2780-157 Oeiras</wicri:noRegion></affiliation></author><author><name sortKey="Fontes, Pedro" sort="Fontes, Pedro" uniqKey="Fontes P" first="Pedro" last="Fontes">Pedro Fontes</name><affiliation wicri:level="1"><nlm:affiliation>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica</wicri:regionArea><wicri:noRegion>2829-516 Monte de Caparica</wicri:noRegion></affiliation></author><author><name sortKey="Roma Rodrigues, Catarina" sort="Roma Rodrigues, Catarina" uniqKey="Roma Rodrigues C" first="Catarina" last="Roma-Rodrigues">Catarina Roma-Rodrigues</name><affiliation wicri:level="1"><nlm:affiliation>UCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>UCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica</wicri:regionArea><wicri:noRegion>2829-516 Monte de Caparica</wicri:noRegion></affiliation></author><author><name sortKey="Fernandes, Alexandra R" sort="Fernandes, Alexandra R" uniqKey="Fernandes A" first="Alexandra R" last="Fernandes">Alexandra R. Fernandes</name><affiliation wicri:level="1"><nlm:affiliation>UCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>UCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica</wicri:regionArea><wicri:noRegion>2829-516 Monte de Caparica</wicri:noRegion></affiliation></author><author><name sortKey="Van Driessche, Gonzalez" sort="Van Driessche, Gonzalez" uniqKey="Van Driessche G" first="Gonzalez" last="Van Driessche">Gonzalez Van Driessche</name><affiliation wicri:level="1"><nlm:affiliation>UCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>UCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica</wicri:regionArea><wicri:noRegion>2829-516 Monte de Caparica</wicri:noRegion></affiliation></author><author><name sortKey="Devreese, Bart" sort="Devreese, Bart" uniqKey="Devreese B" first="Bart" last="Devreese">Bart Devreese</name><affiliation wicri:level="3"><nlm:affiliation>Laboratory for Protein Biochemistry and Biomolecular Engineering, Department of Biochemistry and Microbiology, Gent University, 9000 Gent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory for Protein Biochemistry and Biomolecular Engineering, Department of Biochemistry and Microbiology, Gent University, 9000 Gent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName></affiliation></author><author><name sortKey="Moura, Isabel" sort="Moura, Isabel" uniqKey="Moura I" first="Isabel" last="Moura">Isabel Moura</name><affiliation wicri:level="1"><nlm:affiliation>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica</wicri:regionArea><wicri:noRegion>2829-516 Monte de Caparica</wicri:noRegion></affiliation></author><author><name sortKey="Moura, Jose J G" sort="Moura, Jose J G" uniqKey="Moura J" first="José J G" last="Moura">José J G. Moura</name><affiliation wicri:level="1"><nlm:affiliation>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica</wicri:regionArea><wicri:noRegion>2829-516 Monte de Caparica</wicri:noRegion></affiliation></author><author><name sortKey="Almeida, M Gabriela" sort="Almeida, M Gabriela" uniqKey="Almeida M" first="M Gabriela" last="Almeida">M Gabriela Almeida</name><affiliation wicri:level="1"><nlm:affiliation>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal; Centro de Investigação Interdisciplinar Egas Moniz (CiiEM), Instituto Superior de Ciências da Saúde Egas Moniz, Campus Universitário, Quinta da Granja, 2829-511 Caparica, Portugal. Electronic address: mg.almeida@fct.unl.pt.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal; Centro de Investigação Interdisciplinar Egas Moniz (CiiEM), Instituto Superior de Ciências da Saúde Egas Moniz, Campus Universitário, Quinta da Granja, 2829-511 Caparica</wicri:regionArea><wicri:noRegion>2829-511 Caparica</wicri:noRegion></affiliation></author></analytic><series><title level="j">Biochimica et biophysica acta. Proteins and proteomics</title><idno type="ISSN">1570-9639</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anaerobiosis (genetics)</term><term>Bacterial Proteins (genetics)</term><term>Bacterial Proteins (metabolism)</term><term>Culture Media (chemistry)</term><term>Culture Media (pharmacology)</term><term>Desulfovibrio desulfuricans (drug effects)</term><term>Desulfovibrio desulfuricans (genetics)</term><term>Desulfovibrio desulfuricans (growth & development)</term><term>Desulfovibrio desulfuricans (metabolism)</term><term>Electron Transport (MeSH)</term><term>Electrons (MeSH)</term><term>Electrophoresis, Gel, Two-Dimensional (MeSH)</term><term>Gene Expression Regulation, Bacterial (MeSH)</term><term>Gene Ontology (MeSH)</term><term>Metabolic Networks and Pathways (MeSH)</term><term>Molecular Sequence Annotation (MeSH)</term><term>Nitrate Reductase (genetics)</term><term>Nitrate Reductase (metabolism)</term><term>Nitrates (metabolism)</term><term>Nitrates (pharmacology)</term><term>Nitrite Reductases (genetics)</term><term>Nitrite Reductases (metabolism)</term><term>Oxidation-Reduction (MeSH)</term><term>Proteome (genetics)</term><term>Proteome (metabolism)</term><term>Sulfates (metabolism)</term><term>Sulfates (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anaérobiose (génétique)</term><term>Annotation de séquence moléculaire (MeSH)</term><term>Desulfovibrio desulfuricans (croissance et développement)</term><term>Desulfovibrio desulfuricans (effets des médicaments et des substances chimiques)</term><term>Desulfovibrio desulfuricans (génétique)</term><term>Desulfovibrio desulfuricans (métabolisme)</term><term>Gene Ontology (MeSH)</term><term>Milieux de culture (composition chimique)</term><term>Milieux de culture (pharmacologie)</term><term>Nitrate reductase (génétique)</term><term>Nitrate reductase (métabolisme)</term><term>Nitrates (métabolisme)</term><term>Nitrates (pharmacologie)</term><term>Nitrite reductases (génétique)</term><term>Nitrite reductases (métabolisme)</term><term>Oxydoréduction (MeSH)</term><term>Protéines bactériennes (génétique)</term><term>Protéines bactériennes (métabolisme)</term><term>Protéome (génétique)</term><term>Protéome (métabolisme)</term><term>Régulation de l'expression des gènes bactériens (MeSH)</term><term>Sulfates (métabolisme)</term><term>Sulfates (pharmacologie)</term><term>Transport d'électrons (MeSH)</term><term>Voies et réseaux métaboliques (MeSH)</term><term>Électrons (MeSH)</term><term>Électrophorèse bidimensionnelle sur gel (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>Nitrate Reductase</term><term>Nitrite Reductases</term><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Milieux de culture</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Desulfovibrio desulfuricans</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Desulfovibrio desulfuricans</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Desulfovibrio desulfuricans</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Anaerobiosis</term><term>Desulfovibrio desulfuricans</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Desulfovibrio desulfuricans</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Anaérobiose</term><term>Desulfovibrio desulfuricans</term><term>Nitrate reductase</term><term>Nitrite reductases</term><term>Protéines bactériennes</term><term>Protéome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Bacterial Proteins</term><term>Desulfovibrio desulfuricans</term><term>Nitrate Reductase</term><term>Nitrates</term><term>Nitrite Reductases</term><term>Proteome</term><term>Sulfates</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Desulfovibrio desulfuricans</term><term>Nitrate reductase</term><term>Nitrates</term><term>Nitrite reductases</term><term>Protéines bactériennes</term><term>Protéome</term><term>Sulfates</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Milieux de culture</term><term>Nitrates</term><term>Sulfates</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Culture Media</term><term>Nitrates</term><term>Sulfates</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electron Transport</term><term>Electrons</term><term>Electrophoresis, Gel, Two-Dimensional</term><term>Gene Expression Regulation, Bacterial</term><term>Gene Ontology</term><term>Metabolic Networks and Pathways</term><term>Molecular Sequence Annotation</term><term>Oxidation-Reduction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Annotation de séquence moléculaire</term><term>Gene Ontology</term><term>Oxydoréduction</term><term>Régulation de l'expression des gènes bactériens</term><term>Transport d'électrons</term><term>Voies et réseaux métaboliques</term><term>Électrons</term><term>Électrophorèse bidimensionnelle sur gel</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Sulfate-reducing bacteria (SRB) are a diverse group of anaerobic microorganisms that obtain their energy from dissimilatory sulfate reduction. Some SRB species have high respiratory versatility due to the possible use of alternative electron acceptors. A good example is Desulfovibrio desulfuricans ATCC 27774, which grows in the presence of nitrate (end product: ammonium) with higher rates and yields to those observed in sulfate containing medium (end product: sulfide). In this work, the mechanisms supporting the respiratory versatility of D. desulfuricans were unraveled through the analysis of the proteome of the bacterium under different experimental conditions. The most remarkable difference in the two-dimensional gel electrophoresis maps is the high number of spots exclusively represented in the nitrate medium. Most of the proteins with increase abundance are involved in the energy metabolism and the biosynthesis of amino acids (or proteins), especially those participating in ammonium assimilation processes. qPCR analysis performed during different stages of the bacterium's growth showed that the genes involved in nitrate and nitrite reduction (napA and nrfA, respectively) have different expressions profiles: while napA did not vary significantly, nrfA was highly expressed at a 6h time point. Nitrite levels measured along the growth curve revealed a peak at 3h. Thus, the initial consumption of nitrate and concomitant production of nitrite must induce nrfA expression. The activation of alternative mechanisms for energy production, aside several N-assimilation metabolisms and detoxification processes, solves potential survival problems in adapting to different environments and contributes to higher bacterial growth rates.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28847524</PMID><DateCompleted><Year>2018</Year><Month>02</Month><Day>08</Day></DateCompleted><DateRevised><Year>2018</Year><Month>09</Month><Day>17</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1570-9639</ISSN><JournalIssue CitedMedium="Print"><Volume>1865</Volume><Issue>11 Pt A</Issue><PubDate><Year>2017</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Biochimica et biophysica acta. Proteins and proteomics</Title><ISOAbbreviation>Biochim Biophys Acta Proteins Proteom</ISOAbbreviation></Journal><ArticleTitle>Understanding the response of Desulfovibrio desulfuricans ATCC 27774 to the electron acceptors nitrate and sulfate - biosynthetic costs modulate substrate selection.</ArticleTitle><Pagination><MedlinePgn>1455-1469</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1570-9639(17)30197-8</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.bbapap.2017.07.021</ELocationID><Abstract><AbstractText>Sulfate-reducing bacteria (SRB) are a diverse group of anaerobic microorganisms that obtain their energy from dissimilatory sulfate reduction. Some SRB species have high respiratory versatility due to the possible use of alternative electron acceptors. A good example is Desulfovibrio desulfuricans ATCC 27774, which grows in the presence of nitrate (end product: ammonium) with higher rates and yields to those observed in sulfate containing medium (end product: sulfide). In this work, the mechanisms supporting the respiratory versatility of D. desulfuricans were unraveled through the analysis of the proteome of the bacterium under different experimental conditions. The most remarkable difference in the two-dimensional gel electrophoresis maps is the high number of spots exclusively represented in the nitrate medium. Most of the proteins with increase abundance are involved in the energy metabolism and the biosynthesis of amino acids (or proteins), especially those participating in ammonium assimilation processes. qPCR analysis performed during different stages of the bacterium's growth showed that the genes involved in nitrate and nitrite reduction (napA and nrfA, respectively) have different expressions profiles: while napA did not vary significantly, nrfA was highly expressed at a 6h time point. Nitrite levels measured along the growth curve revealed a peak at 3h. Thus, the initial consumption of nitrate and concomitant production of nitrite must induce nrfA expression. The activation of alternative mechanisms for energy production, aside several N-assimilation metabolisms and detoxification processes, solves potential survival problems in adapting to different environments and contributes to higher bacterial growth rates.</AbstractText><CopyrightInformation>Copyright © 2017 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sousa</LastName><ForeName>Joana R</ForeName><Initials>JR</Initials><AffiliationInfo><Affiliation>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Silveira</LastName><ForeName>Célia M</ForeName><Initials>CM</Initials><AffiliationInfo><Affiliation>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal; Instituto de Tecnologia Química e Biológica António Xavier, Universidade NOVA de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fontes</LastName><ForeName>Pedro</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Roma-Rodrigues</LastName><ForeName>Catarina</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>UCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fernandes</LastName><ForeName>Alexandra R</ForeName><Initials>AR</Initials><AffiliationInfo><Affiliation>UCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Van Driessche</LastName><ForeName>Gonzalez</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>UCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Devreese</LastName><ForeName>Bart</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Laboratory for Protein Biochemistry and Biomolecular Engineering, Department of Biochemistry and Microbiology, Gent University, 9000 Gent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Moura</LastName><ForeName>Isabel</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Moura</LastName><ForeName>José J G</ForeName><Initials>JJG</Initials><AffiliationInfo><Affiliation>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Almeida</LastName><ForeName>M Gabriela</ForeName><Initials>MG</Initials><AffiliationInfo><Affiliation>UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal; Centro de Investigação Interdisciplinar Egas Moniz (CiiEM), Instituto Superior de Ciências da Saúde Egas Moniz, Campus Universitário, Quinta da Granja, 2829-511 Caparica, Portugal. Electronic address: mg.almeida@fct.unl.pt.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>08</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Biochim Biophys Acta Proteins Proteom</MedlineTA><NlmUniqueID>101731734</NlmUniqueID><ISSNLinking>1570-9639</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009566">Nitrates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013431">Sulfates</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.7.-</RegistryNumber><NameOfSubstance UI="D009572">Nitrite Reductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.7.99.4</RegistryNumber><NameOfSubstance UI="D050901">Nitrate Reductase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000693" MajorTopicYN="N">Anaerobiosis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045285" MajorTopicYN="N">Desulfovibrio desulfuricans</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004579" MajorTopicYN="N">Electron Transport</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004583" MajorTopicYN="Y">Electrons</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015180" MajorTopicYN="N">Electrophoresis, Gel, Two-Dimensional</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015964" MajorTopicYN="Y">Gene Expression Regulation, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D063990" MajorTopicYN="N">Gene Ontology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053858" MajorTopicYN="N">Metabolic Networks and Pathways</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D058977" MajorTopicYN="N">Molecular Sequence Annotation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050901" MajorTopicYN="N">Nitrate Reductase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009566" MajorTopicYN="N">Nitrates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009572" MajorTopicYN="N">Nitrite Reductases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013431" MajorTopicYN="N">Sulfates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Ammonium assimilation</Keyword><Keyword MajorTopicYN="Y">Desulfovibrio desulfuricans ATCC 27774</Keyword><Keyword MajorTopicYN="Y">Energy metabolism</Keyword><Keyword MajorTopicYN="Y">Nitrate reduction</Keyword><Keyword MajorTopicYN="Y">Proteomics</Keyword><Keyword MajorTopicYN="Y">Respiratory flexibility</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>04</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>07</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>07</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>8</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>2</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>8</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28847524</ArticleId><ArticleId IdType="pii">S1570-9639(17)30197-8</ArticleId><ArticleId IdType="doi">10.1016/j.bbapap.2017.07.021</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li><li>Portugal</li></country><region><li>Province de Flandre-Orientale</li></region><settlement><li>Gand</li></settlement></list><tree><country name="Portugal"><noRegion><name sortKey="Sousa, Joana R" sort="Sousa, Joana R" uniqKey="Sousa J" first="Joana R" last="Sousa">Joana R. Sousa</name></noRegion><name sortKey="Almeida, M Gabriela" sort="Almeida, M Gabriela" uniqKey="Almeida M" first="M Gabriela" last="Almeida">M Gabriela Almeida</name><name sortKey="Fernandes, Alexandra R" sort="Fernandes, Alexandra R" uniqKey="Fernandes A" first="Alexandra R" last="Fernandes">Alexandra R. Fernandes</name><name sortKey="Fontes, Pedro" sort="Fontes, Pedro" uniqKey="Fontes P" first="Pedro" last="Fontes">Pedro Fontes</name><name sortKey="Moura, Isabel" sort="Moura, Isabel" uniqKey="Moura I" first="Isabel" last="Moura">Isabel Moura</name><name sortKey="Moura, Jose J G" sort="Moura, Jose J G" uniqKey="Moura J" first="José J G" last="Moura">José J G. Moura</name><name sortKey="Roma Rodrigues, Catarina" sort="Roma Rodrigues, Catarina" uniqKey="Roma Rodrigues C" first="Catarina" last="Roma-Rodrigues">Catarina Roma-Rodrigues</name><name sortKey="Silveira, Celia M" sort="Silveira, Celia M" uniqKey="Silveira C" first="Célia M" last="Silveira">Célia M. Silveira</name><name sortKey="Van Driessche, Gonzalez" sort="Van Driessche, Gonzalez" uniqKey="Van Driessche G" first="Gonzalez" last="Van Driessche">Gonzalez Van Driessche</name></country><country name="Belgique"><region name="Province de Flandre-Orientale"><name sortKey="Devreese, Bart" sort="Devreese, Bart" uniqKey="Devreese B" first="Bart" last="Devreese">Bart Devreese</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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